GoldCyto Sperm DNA kit
KIT TO ANALYZE SPERM DNA FRAGMENTATION
The GoldCyto Sperm òKit from Goldcyto Biotech corp. is a simple test that allows assessment of sperm DNA fragmentation in human.
The method is based on the Sperm Chromatin Dispersion (SCD) test (Fernández et al., J. Androl 24:59–66, 2003; Fertil Steril 84:833-842, 2005). Intact unfixed spermatozoa (fresh, frozen/unthawed, diluted or neat samples) are immersed in an inert agarose microgel on a pretreated slide. An initial acid treatment denatures DNA in those sperm cells with fragmented DNA. Following this, the lysing solution removes most of the nuclear proteins, and in the absence of massive DNA breakage produces nucleoids with large halos of spreading DNA loops, emerging from a central core. However, the nucleoids from spermatozoa with fragmented DNA either do not show a dispersion halo or the halo is minimal.
*DESCRIPTION OF KIT CONTENTS
-20 Pretreated slides
-20 Eppendorf tubes with low-melting-point agarose(Agarose Cell Support)
-1 Bottle of Phosphate Buffer Saline.50 ml
-1 Bottle of Denaturant Agent.100 ml
-1 Bottle of lysis solution.100 ml
Catalogue ID | Description | Tests/kit |
GC-SCD-10 | DNA fragment SCD test kit | 10T |
GC-SCD-20 | DNA fragment SCD test kit | 20T |
